Papers from 2005

Below are a number of refereed papers from 2005 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include research and testing in nutrition, medicine, biomonitoring and genetic toxicology:

Biocompatibility In Vitro Tests of Mineral Trioxide Aggregate and Regular and White Portland Cements
(2005) D.A. Ribeiro et al, Journal of Endodontics 31(8):605-607

The novel human gene aprataxin is directly involved in DNA single-strand-break repair
(2005) P. Mosesso et al, Cellular and Molecular Life Sciences 62(4):485-491

Genetic polymorphisms and the effect of cigarette smoking in the comet assay
(2005) H. Hoffmann et al, Mutagenesis 20(5):359-364

The green tea polyphenol (-)-epigallocatechin gallate and green tea can protect human cellular DNA from ultraviolet and visible radiation-induced damage
(2005) N. Morley et al, Photodermatology, Photoimmunology & Photomedicine 21(1):15-22

Radiosensitivity of lymphoblastoid cell lines with a heterozygous BRCA1 mutation is not detected by the comet assay and pulsed field gel electrophoresis
(2005) K. Trenz, P. Schütz and G. Speit, Mutagenesis 20(2):131-137

Effects of pesticides on human peripheral lymphocytes in vitro: induction of DNA damage
(2005) Ü. Ündeger and N. Basaran, Archives of Toxicology 79(3):169-176

Assessment of genetic damage induced by dental bleaching agents on mouse lymphoma cells by single cell gel (comet) assay
(2005) D.A. Ribeiro, M.E.A. Marques and D.M.F. Salvadori, Journal of Oral Rehabilitation 32(10):766–771

Investigation into possible DNA damaging effects of ultrasound in occupationally exposed medical personnel - the alkaline comet assay study
(2005) V. Garaj-Vrhovac and N. Kopjar, Journal of Applied Toxicology 25(3):184-192

In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate
(2005) D.A. Ribeiro et al, Brazilian Oral Research 19(3):183-187

Involvement of oxygen free radicals in the serum-mediated increase of benzoquinone genotoxicity
(2005) R. Fabiani, A. De Bartolomeo and G. Morozzi, Environmental and Molecular Mutagenesis 46(3):156-163

Transcription-Associated Breaks in Xeroderma Pigmentosum Group D Cells from Patients with Combined Features of Xeroderma Pigmentosum and Cockayne Syndrome
(2005) T. Theron et al, Molecular and Cellular Biology 25(18):8368-8378

Rehabilitation Decreases Exercise-induced Oxidative Stress in Chronic Obstructive Pulmonary Disease
(2005) E.M. Mercken et al, American Journal of Respiratory and Critical Care Medicine 172:994-1001

Nitrite enhances neutrophil-induced DNA strand breakage in pulmonary epithelial cells by inhibition of myeloperoxidase
(2005) A.M. Knaapen et al, Carcinogenesis 26(9):1642-1648

Metal-specific differences in levels of DNA damage caused by synovial fluid recovered at revision arthroplasty
(2005) A.P. Davies et al, Journal of Bone and Joint Surgery 87-B(10):1439-1444

Inhibition of cell growth and induction of apoptosis in human prostate cancer cell lines by 6-aminoquinolone WM13
(2005) A. Minelli et al, Oncology reports 13(6):1113-20

DNA damage and G2/M arrest in Syrian hamster embryo cells during Malachite green exposure are associated with elevated phosphorylation of ERK1 and JNK1
(2005) B. Bose, L. Motiwale, K. Rao, Cancer Letters 230(2):260-270

Dose – response for radiation-induced apoptosis, residual 53BP1 foci and DNA-loop relaxation in human lymphocytes
(2005) J.Torudd et al, International Journal of Radiation Biology 81(2):125-138

Single and repeated moderate consumption of native or dealcoholized red wine show different effects on antioxidant parameters in blood and DNA strand breaks in peripheral leukocytes in healthy volunteers: a randomized controlled trial
(2005) B.M. Arendt et al, Nutrition Journal 4(33)

Ochratoxin A induces oxidative DNA damage in liver and kidney after oral dosing to rats
(2005) H.G. Kamp et al, Molecular Nutrition & Food Research 49(12):1160-1167

Chromatin supraorganization, DNA fragmentation, and cell death in snake erythrocytes.
(2005) M. Miyamoto et al, Biochemistry and cell biology 83(1):15-27

Modulation of endothelial and smooth muscle function by bed rest and hypoenergetic, low-fat nutrition
(2005) C. Hesse et al, Journal of Applied Physiology 99:2196-2203

Genotoxicity of 4-Hydroxy-2-Nonenal in Human Colon Tumor Cells Is Associated with Cellular Levels of Glutathione and the Modulation of Glutathione S-Transferase A4 Expression by Butyrate
(2005) N. Knoll et al, Toxicological Sciences 86(1):27-35

Polyphenolic apple juice extracts and their major constituents reduce oxidative damage in human colon cell lines
(2005) S.Schaefer et al, Molecular Nutrition & Food Research 50(1):24-33

Cloudy apple juice decreases DNA damage, hyperproliferation and aberrant crypt foci development in the distal colon of DMH-initiated rats
(2005) S.W. Barth et al, Carcinogenesis 26(8):1414-1421

Quotes

It is, indeed, much quicker than the old image analysis system that we had. We are very pleased with it.

Senior Researcher, Public Health Agency, Finland

We are really very happy with the comet analysis system.

Research Scientist, University, Austria

Everything has gone very well with the system and we are very pleased with it.

Professor, University, UK

All is going well and the more I use the software the more I love the way it's so simple and intuitive...the comet software is running well and in increasing use.

Scientist, University, UK

The system is working great.

Senior Research Scientist, Cancer Research Institute, UK

We started using Comet Assay IV and it works well...

Researcher, Nuclear Agency, France

We are very satisfied with your software.

Scientist, Cancer Research Institute, Slovakia

Everything has gone very well with the system and we are very pleased with it.

Scientist, University, UK

It is actually working great.

Professor, University, USA

Just to let you know we have started the validation study for Comet Assay IV and it has been going very well! We ran a comet assay last week and scored the slides with no problems at all. I am very pleased with the system and it will certainly make a difference when we run some comet studies this year!

Study Director, Food & Household Products Company, UK

Perfect and very clear installation and training!

Researcher, Contract Testing Laboratory, Europe

I have been using the Comet Assay IV Software. It is great! During my software validation, I realized how much easier and precise it is compared to our old software. It makes analyzing the comet slides more enjoyable

Scientist, Medical Research Centre, USA

Thanks for your attention and great service. I am so glad that we have Comet IV.

Study Director, Contract Research Organisation, USA

We are impressed indeed! It has saved us quite a lot of time and pains.

Director, Toxicology Research Centre, Spain

...and the system is running great.

Medical Researcher, Cancer Research Institute, USA

I love the software. Keep up the good work over there.

Study Director, Contract Research Organisation, USA

We are very happy with everything, it is easy to work with your software.

Senior Scientist, National Health Institute, Portugal

So it's working out fine and we're very happy!

Toxicologist, Health Agency, Canada

Comet Assay IV software is in use. Currently our comet analysis is based on tail moment measurement and I must say that everything works properly.

Researcher, Cancer Research Institute, Slovakia

We have just recently utilized the system and are extremely pleased.

Assistant Professor, University, USA

...Everything is fine with the Comet Assay IV system...and I'm pretty happy about this new software with just clicking on the cells instead of drawing a frame around them

Researcher, Cancer Research Organisation, UK

Excellent, perfectly straightforward.

Scientist, University, UK

The system was set up last month, and so far it works very well. Both my boss and I are very satisfied with it and like it very much.

Researcher, University Hospital, Germany

The Comet Assay IV system we already installed and is working perfectly.

Scientist, Environmental Institute, Norway

We're now using the software and it works great. It has cut our time for comets by at least half.

Post Doctoral Fellow, National Institute of Health, USA

I don't have any questions about the software as it has worked quite well for us...so far we are very happy with the software.

Research Scientist, University, Australia

Total change in our lab since you came (with Comet Assay IV of course...)! thanks... :-)

Postgraduate Researcher, University, France

We have installed the software and are using it without any problem. It's really simple! My PhD students are very happy with it.

Professor, University, Spain

Perfect and very clear installation and training.

Study Director, Contract Research Organisation, Europe

The system is up and running. Everybody is impressed with the speed of scoring slides.

Researcher, University, South Africa

It's an amazing program! Really useful!

Research Scientist, University, Brazil

I'm really a fan of your system :)

Genetic Toxicologist, Global Pharmaceutical Company

We are using the Comet Assay IV a lot (and our use is increasing). It's working very well.

Professor, Children's Research Hospital, USA

The current Comet Assay IV system works without any trouble…we are pleased with the system.

Scientist, Chemical Company, Japan

Yes, it is very useful and we are in the process of writing a paper also from the data derived from Comet Assay IV.

Assistant Professor, University, USA

Excellent, the training was very helpful and specific to in-house technicalities.

Lecturer, University, UK

...we are "happy users" of the software

Senior Researcher, Risk Assessment Institute, Germany

So far, I'm very happy with the system.

Research Lecturer, University, UK

We have found the Comet Assay IV software very useful and convenient.

Research Scientist, Food Safety Agency, France

The installation was quick and all the details were described and easily understandable for the technicians.

Head of Department, Global Chemical Company, Germany

We have had no problems so far, and we are very pleased with the system.

Senior Researcher, Public Health Agency, Finland