Comet Assay IV Publications
Perceptive Instruments Comet Assay IV image analysis systems have been sited within peer-reviewed publications hundreds of times. Here, we have listed a few of them:
Papers from 2019
Spatial and temporal variation of three biomarkers in Mytilus edulis
S Strubbia, BP Lyons, RJ Lee - Marine Pollution Bulletin, 2019
...The tails were evaluated through Comet Assay IV analysis software (Perceptive Instruments Ltd) and Camera AVT smart view for, Fire Package version 1.6.2) to measure the total image fluorescence intensity, head intensity, tail intensity, head length and tail length. The results were recorded as the % tail intensity over 50 cells...
Poly (e-caprolactone) microspheres for prolonged release of selenium nanoparticles
N Filipovic, L Veselinovic, S Razic, S Jeremic, M Filipic… - Materials Science and Engineering, 2019
... Images of 50 randomly selected nuclei per experimental point were analyzed with image analysis software Comet Assay IV (Perceptive Instruments, UK). The percentages of tail DNA were used to measure the levels of DNA damage …
Papers from 2018
Chemopreventive potential of in vitro fermented raw and roasted hazelnuts in LT97 colon adenoma cells
M Glei, S Fischer, J Lamberty, D Ludwig… - Anticancer Research, 2018
… After staining DNA with SYBR® Green (Sigma Aldrich, Munich, Germany) DNA damage was detected using a fluorescence microscope (ZEISS Axiostar plus; Carl Zeiss Jena GmbH) and image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK) …
Comprehensive evaluation of the flavonol anti-oxidants, alpha-glycosyl isoquercitrin and isoquercitrin, for genotoxic potential
CA Hobbs, M Koyanagi, C Swartz, J Davis, S Kasamoto… - Food and Chemical Toxicology …, 2018
… slides with SYBR Gold™ (Molecular Probes, Invitrogen, Carlsbad, CA), 150 cells were scored per sample at 200× total magnification without scorers' knowledge of animal identity using Comet Assay IV Imaging Software, Version 4.3.1 (Perceptive Instruments, Ltd., Suffolk, UK) …
Papers from 2017
Genotoxicity and gene expression analyses of liver and lung tissues of mice treated with titanium dioxide nanoparticles
Y Li, J Yan, W Ding, Y Chen, LM Pack, T Chen - Mutagenesis, 2017
... random from each slide. Scoring was performed on a platform consisting of a Nikon 50i fluorescent microscope (Nikon, USA) and Comet IV digital imaging software (Perceptive Instruments, Wiltshire, UK). Percent (%) DNA in ...
EGCG Prevents High Fat Diet-Induced Changes in Gut Microbiota, Decreases of DNA Strand Breaks, and Changes in Expression and DNA Methylation of Dnmt1 and …
M Remely, F Ferk, S Sterneder, T Setayesh, S Roth… - Oxidative Medicine and …, 2017
... Slides were examined under a fluorescence microscope (Nikon EFD-3, Japan) using 25-fold magnification. DNA migration was determined with a computer aided comet assay image analysis system (Comet Assay IV, Perceptive Instruments, UK). 2.2. Gene Expression Analysis. ...
Papers from 2016
Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3
S Kolarevic, M Kracun-Kolarevic, J Kostic, J Slobodnik… - Science of The Total Environment, 2016
... The slides were examined with a fluorescence microscope (Leica, DMLS, Austria, under magnification 400 ×, excitation filter 510–560 nm, barrier filter 590 nm). Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). ...
In vitro comet and micronucleus assays do not predict morphological transforming effects of silica particles in Syrian Hamster Embryo cells.
C Darne, C Coulais, F Terzetti, C Fontana… -Genetic Toxicology and Environmental Mutagensis 2016
... The slides were then washed with Tris–HCl 0.4 M for 15 min and stained with propidium iodide. Images of 100 randomly selected comets were analysed from each sample, using specific image analyser software (Comet assay IV, Perceptive Instruments, United Kingdom ...
Papers from 2015
Recommendations for increasing alkaline comet assay reliability in plants
B Pourrut, E Pinelli, VC Mendiola, J Silvestre, F Douay - Mutagenesis, 2015
... For each slide, 50 randomly selected nuclei were analysed using an Olympus fluorescence microscope with an excitation filter of 510–560nm and a barrier of 590nm. A computerized image analysis system (Comet Assay IV; Perceptive Instruments) was employed. ...
DNA repair after X-irradiation: lessons from plants
J Einset, AR Collins - Mutagenesis, 2015
... Analysis of comets was performed after staining with 1 µg/ml 4'-6-diamidino-2-phenylindole using a Nikon Eclipse TS100 fluorescence microscope equipped with an Allied Vision Technologies camera and the Comet Assay IV image analysis program (Perceptive Instruments). ...
Papers from 2014
Unbiased compound screening identifies unexpected drug sensitivities and novel treatment options for gastrointestinal stromal tumors
S Boichuk, DJ Lee, KR Mehalek, KR Makielski… - Cancer Research, 2014
... DNA was labeled with SYBR Green and slides were viewed under a Nikon ECLIPSE 50i epifluorescence microscope (Nikon). Comet tails were analyzed using Comet Assay IV v4.2 software (Perceptive Instruments). At least 100 cells were analyzed per sample. ...
Is the antioxidative effectiveness of a bilberry extract influenced by encapsulation?
M Baum, M Schantz, S Leick, S Berg, M Betz, K Frank… - … of the Science of Food and …, 2014
... Slides were analysed by computerized image analysis (Comet IV, Perceptive Instruments. DNA damage was calculated as the tail intensity (TI%: DNA in the comet tail as a percent of total DNA). ... 20. Schantz M, Baum M and Richling E, Antioxidative efficiency of an anthocyanin ...
Papers from 2013
Evidence for different mechanisms of action behind the mutagenic effects of 4-NOPD and OPD: the role of DNA damage, oxidative stress and an imbalanced …
R Åsgård, S Haghdoost, SO Golkar, B Hellman… - Mutagenesis, 2013
... Before the image analysis, the slides were stained with ethidium bromide (30 µl/slide; 200mg/ml) and analysed in a fluorescence microscope using the image analysis software Comet Assay IV (Perceptive Instruments, UK). ...
Antioxidant and genoprotective effects of spent coffee extracts in human cells
J Bravo, L Arbillaga, M Paz de Peña, C Cid - Food and Chemical Toxicology, 2013
... NIKON). A total of 100 comets on each slide were scored through a computer-assisted image analysis (Comet assay IV, Perceptive Instruments), by measuring the percentage of DNA in the tail. 2.7. Statistical analysis. Data are ...
Papers from 2012
Cryopreservation and storage of mussel (Mytilus spp.) hemocytes for latent analysis by the Comet assay
A Kwok, BP Lyons, NJ Hodges, TP Bean - Mutation Research/Genetic Toxicology and ..., 2012
... DNA damage was expressed as the % tail intensity. The images were measured with Comet Assay IV (Perceptive Instruments), and data were compiled with Comet Assay Spreadsheet Generator Version 1-3-1 (Perceptive Instruments). 2.7. Data Analysis. ...
Age and gender effects on DNA strand break repair in peripheral blood mononuclear cells
C Garm, M Moreno-Villanueva, A Bürkle, I Petersen… - Aging Cell, 2012
... DNA was stained using SYBR Gold (Invitrogen) and analysis was performed using a fluorescent microscope (Leica DMLB) equipped with camera (Leica DC 200). Live quantification was performed on 60-100 cells from each gel using Perceptive Instruments IV software. ...
Papers from 2011
A protocol for the evaluation of genotoxicity in bile of carp (Cyprinus carpio) exposed to lake water treated with different disinfectants
(2011) M. Villarini et al, Chemosphere 84(10):1521-1526
Acellular comet assay: a tool for assessing variables influencing the alkaline comet assay
(2011) E Kennedy et al, Radiat Prot Dosimetry (E-pub ahead of print)
Acrylamide genotoxicity in young versus adult gpt delta male rats
(2011) N. Koyama et al, Mutagenesis 26(4):545-549
Papers from 2010
A multibiomarker approach in Coris julis living in a natural environment
(2010) S. Fasulo et al, Ecototoxicol. and Environ. Safety
An ECVAG dagger trial on assessment of oxidative damage to DNA measured by the comet assay
(2010) C Johansson et al, Mutagenesis 25(2):125-132
Anomalous genotoxic responses induced in mouse lymphoma L5178Y cells by potassium bromate
(2010) C. Priestley et al, Toxicology 267(1-3):45-53
Papers from 2009
Below are a number of refereed papers from 2009 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include research and testing in nutrition, medicine, biomonitoring and genetic toxicology:
Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells
(2009) K. Gerloff et al, Nanotoxicology 3(4): 355-364
Delphinidin Modulates the DNA-Damaging Properties of Topoisomerase II Poisons
(2009) M. Esselen et al, Chem. Res. Toxicol. 22(3):554-564
Genotoxicity evaluation of locally produced dental porcelain – An in vitro study using the Ames and Comet assays
(2009) M. Noushad et al., Toxicol In Vitro. 23(6):1145-50
Papers from 2008
Below are a number of refereed papers from 2008 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include research and testing in nutrition, medicine, biomonitoring and genetic toxicology:
Alcohol induces DNA damage and the Fanconi anemia D2 protein implicating FANCD2 in the DNA damage response pathways in brain
(2008) S.L. Rulten et al, Alc. Clin. Exp. Res. 32(7):1186-1196
Analysis of DNA breaks, DNA damage response, and apoptosis produced by high NaCl
(2008) N.I. Dmitrieva et al, Am. J. Phys. Renal Phys. 295(1):1678-1688
Anthocyanin/Polyphenolic–Rich Fruit Juice Reduces Oxidative Cell Damage in an Intervention Study with Patients on Hemodialysis
(2008) T.M. Spormann et al, Canc. Epi. Biom. & Prev. 17(1):3372-3380
Papers from 2007
Below are a number of refereed papers from 2007 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include potentially harmful and beneficial dietary factors; biomonitoring; molecular epidemiology; photobiology; mechanistic studies, clinical and dentistry.
8-Oxoguanine DNA Glycosylase and MutY Homolog Are Involved in the Incision of Arsenite-Induced DNA Adducts
(2007) Y. Pu et al, Tox. Sci. 95(2):376-382
Absence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats
(2007) A. de Lima Leite et al, Human & Expt. Tox. 26(5):435-440
An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans
(2007) I. H. Ismail et al, Nuc. Acids Res. 35(5):e36
Papers from 2006
Below are a number of refereed papers from 2006 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include genotoxicity testing of materials used in dentistry; potentially harmful and beneficial dietary factors; biomonitoring; molecular epidemiology; photobiology; mechanistic studies and clinical.
2-Dodecylcyclobutanone, a radiolytic product of palmitic acid, is genotoxic in primary human colon cells and in cells from preneoplastic lesions
(2006) N. Knoll et al, Mut. Res. 594(1-2):10-19
A Protease Pathway for the Repair of Topoisomerase II-DNA Covalent Complexes
(2006) A. Zhang et al, Journal of Biological Chemistry 281(47):35997-36003
Alkaline comet assay study with breast cancer patients: evaluation of baseline and chemotherapy-induced DNA damage in non-target cells
(2006) N. Kopjar et al, Clin. Exp. Med. 6(4):177-190
Papers from 2005
Below are a number of refereed papers from 2005 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include research and testing in nutrition, medicine, biomonitoring and genetic toxicology:
Biocompatibility In Vitro Tests of Mineral Trioxide Aggregate and Regular and White Portland Cements
(2005) D.A. Ribeiro et al, Journal of Endodontics 31(8):605-607
The novel human gene aprataxin is directly involved in DNA single-strand-break repair
(2005) P. Mosesso et al, Cellular and Molecular Life Sciences 62(4):485-491
Genetic polymorphisms and the effect of cigarette smoking in the comet assay
(2005) H. Hoffmann et al, Mutagenesis 20(5):359-364